Figure 3.

Spherulite^™ stability analysed by gel electrophoresis. The two formulations F1 and F2 were prepared with 5′-radiolabelled oligonucleotide dissolved in H₂O. The spherulites^™ were dispersed in PBS. Vesicle leakage was followed by gel electrophoresis after 10 days at 4°C. On this 10% non-denaturating polyacrylamide gel was loaded the oligonucleotide contained in F2 (lanes 1 and 2) and oligonucleotide contained in F1 (lanes 3 and 4). 10% Triton X-100 was added to both formulations (lanes 2 and 4) to disrupt the vesicles and give the 100% oligonucleotide concentration. (A) Top of the gel, (B) internal marker (59mer) to control the homogeneity of the loading, (C) 5′-radiolabelled encapsulated 22mer PO.