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. 2024 Jan 20;14(3):1224–1240. doi: 10.7150/thno.91119

Figure 3.

Figure 3

CD73 expression on macrophages is induced by IL-6 and regulated by JAK/STAT3 pathway. (A) Relative mRNA expression of Il1a, Il1b, Il6, Il10, Ifna, and Tnfa in senescent LLCs (Se). The indicated results represent the mean ± SEM of 3 independent experiments. (B) Relative mRNA expression of Cd73 in BMDMs after treated with 20 ng/mL different cytokines for 24 h. The indicated results represent the mean ± SEM of 3 independent experiments. (C) Relative mRNA expression of Cd73 in BMDMs after incubation with senescence-conditioned medium (Se CM) and/or 10 μg/mL IL-6-neutralizing antibodies (aIL-6) for 24 h. The indicated results represent the mean ± SEM of 3 independent experiments. (D) Volcano plot showing the upregulated, downregulated, or insignificantly differential expressed genes of BMDMs co-cultured with senescent or control LLCs for 24 h. (E) KEGG pathway enrichment revealing the activation of cytokine-cytokine receptor interaction and JAK/STAT signaling pathways as indicated in BMDMs co-cultured with senescent LLCs. (F) Representative Western blot for protein expression levels of pSTAT3, and STAT3 in BMDMs. (G) Relative mRNA expression of Cd73 in BMDMs after treated with Se CM and/or BP-1-102 (5 μM) for 24 h. The indicated results represent the mean ± SEM of 3 independent experiments. (H) Relative mRNA expression of Cd73 in BMDMs harboring STAT3 siRNAs (siSTAT3) and treated with Se CM for 24 h. The indicated results represent the mean ± SEM of 3 independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not statistically significant.