Figure 6.

Clinical relevance between senescence and CD73⁺ macrophages in lung adenocarcinoma. (A) Representative immunofluorescence staining of senescence-associated marker CDKN2A (red) and CD73 expression (red) on the surface of macrophages (green) in the surgically resected specimens from primary lung adenocarcinoma patients. Scale bar: 20 μm. (B) Linear regression analysis between the frequencies of CDKN2A⁺ senescent cells and CD73⁺CD68⁺ macrophages in specimen sections as described in (A). (C) UMAP plot of single cells colored by the major cell lineages. (D) UMAP plot of myeloid cells, color-coded by clusters and cell subsets as indicated. (E) UMAP plot of NT5E expression in myeloid cells as described in (D). (F) Linear regression analysis between the senescence gene set (SenMayo) expression of tumor cells and the CD73 expression of macrophages in the single-cell dataset. (G) Survival based on the expression level of senescent macrophage gene set in the TCGA lung adenocarcinoma cohort (log-rank test).