Figure 3.

(I) Schematic representation of the production of pH-MBs shows the reversible activation of pH-sensitive microbubbles. (II) The diagrammatic representation of the conjugation of A-HA-SH to mal-MBs and subsequent cross-linking with a pH-sensitive cross-linker to create pH-MBs; (III) pH-MBs imaged with CPS (left) and B-Mode (right) (A) at pH 7.4 and (B) at pH 5 using the in vitro setup shown in (C). (D) Peak CPS signal SD of pH-MBs in neutral (pH 7.4) and acidified (pH 5) conditions (n = 3). The standard deviation is shown by error bars. (E) After initial activation, the activation reversibility of pH-MBs was studied when cycling between pH 5.0 and 7.4. Reproduced with permission from ref. 64. Fig. 3, and Fig. 5 (ACS Publication).