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. 2024 Feb 5;22:57. doi: 10.1186/s12916-024-03264-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — HIF1A regulates trophoblast syncytialization through the MLL1/H3K4me3/TEAD4 axis. A KEGG analyses performed on women at different stages of PE and healthy controls. B HIF1A chromatin immunoprecipitation sequencing (ChIP-seq) signals for MLL1 in cells treated with DMOG or DMSO. C Western blots of MLL1, HIF1A, TEAD4, HCG, and H3K4me3 in BeWo cells treated with a gradient of DMOG concentrations (0, 0.25, and 0.50 mM) and hypoxia exposure time (0, 24, and 48 h). D and E mRNA levels of MLL1, TEAD4, and STB markers in BeWo cells treated with a gradient of DMOG concentrations and hypoxia exposure time. F-J mRNA levels of STB markers (F–H), western blots of MLL1, HIF1A, TEAD4, HCG, and H3K4me3 (I) and immunostainings of E-cadherin (green) and DAPI (blue) (J) in BeWo cells treated with FSK (25 μM) in the absence or presence of DMOG (0.25 mM) and MI-3454 (5 μM). Scale bar, 40 μm. K Quantitative ChIP analysis of HIF1A at the MLL1 promoter in FSK-treated BeWo cells. The values are normalized to IgG. Data are presented as the means ± SD. **P < 0.01, *P < 0.05. DMOG, dimethyloxallyl glycine; HIF1A, hypoxia-inducible factor 1A