Table 1. The efficiency of the homologous recombination with two targeting vectors.
Gene | Size of 5′ flanking region (kb) | Size of 3′ flanking region (kb) | Number of pick-up colony | Number of H.R. colony | Targeting efficiency (%) |
---|---|---|---|---|---|
Epo receptor |
7.0 |
1.1 |
192 |
1 |
0.5 |
EST (AU067162) | 3.0 | 8.0 | 713 | 1 | 0.1 |