Immunofluorescence of peripheral retina sections of the unaffected mother of donor 1 (donor 4) labeled with antibodies to rhodopsin, cone arrestin and red/green cone opsin showed no significant changes in staining of these proteins as compared to control. The unaffected carrier donor retina displayed both rhodopsin (b, green) and red/green cone opsin (d, red) restricted to the photoreceptor outer segments as observed in control retinas (a, c). Cone arrestin labeling in the unaffected carrier donor retina (d, green) displayed similar distribution when compared to the control retina (c). Nuclei were labeled with TO-PRO-3. GCL= ganglion cell layer; INL= inner nuclear layer; ONL= outer nuclear layer; POS = photoreceptor outer segments. Scale bar = 40μm.