TABLE 1.
Quantification of relative expression of endogenous (HisC) and transgenic (12XDWT, 12XPR, or 8XHWT) RD histone genes in 3–6 h old embryos determined by either quantification of band intensities of restriction digested RT-PCR products (PCR data) or by direct sequencing (MiSeq data). A) HisC/HisC; 12XDWT. B) ΔHisC/ΔHisC; 12XPR, 8XHWT. All values are average ± SD for either three (PCR data) or two (MiSeq data) biological replicates. ND = not determined.
| H2A | H2B | H3 | H4 | H1 | ||
|---|---|---|---|---|---|---|
| PCR data | 68.9 ± 5.4 | 80.2 ± 1.8 | 75.4 ± 3.7 | 77.9 ± 4.1 | 80.0 ± 1.1 | HisC |
| 31.1 ± 5.4 | 19.4 ± 1.8 | 24.6 ± 3.7 | 22.2 ± 4.1 | 20.0 ± 1.1 | 12XDWT | |
| MiSeq data | 61.8 ± 2.1 | 74.1 ± 0.7 | 76.7 ± 0.2 | 78.4 ± 0.3 | ND | HisC |
| 39.2 ± 2.0 | 25.9 ± 0.7 | 23.3 ± 0.1 | 21.6 ± 0.2 | ND | 12XDWT |
| PCR data | 63.3 ± 3.7 | 71.5 ± 2.5 | 71.9 ± 5.3 | 63.0 ± 1.5 | 65.6 ± 3.3 | 12XPR |
| 36.7 ± 3.7 | 28.5 ± 2.5 | 28.1 ± 5.3 | 37.0 ± 1.5 | 34.4 ± 3.3 | 8XHWT | |
| MiSeq data | 81.6 ± 0.6 | 77.0 ± 0.1 | 71.5 ± 1.3 | 60.2 ± 2.3 | ND | 12XPR |
| 18.4 ± 0.6 | 23.0 ± 0.1 | 29.5 ± 1.2 | 39.8 ± 2.2 | ND | 8XHWT |