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. Author manuscript; available in PMC: 2024 Feb 6.
Published in final edited form as: Mol Cell. 2022 Jul 18;82(17):3193–3208.e8. doi: 10.1016/j.molcel.2022.06.024

Figure 2. Phase separation is driven by interactions among unfolded barnase molecules.

Figure 2.

(A) Schematic of constructs used for the optoDroplet assay. (B) Representative confocal micrograph images of Neuro2a cells transfected with DDX4 IDR (positive control), WT barnase, and the destabilizing barnase variant (I25A, I96G) optoDroplet constructs before and after light activation. (C) Time lapsed confocal imaging of live Neuro2a cells expressing the I25A, I96G barnase optoDroplet construct. Scale bars in panels B and C correspond to 10 µm.