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. Author manuscript; available in PMC: 2024 Feb 6.
Published in final edited form as: Mol Cell. 2022 Jul 18;82(17):3193–3208.e8. doi: 10.1016/j.molcel.2022.06.024

Figure 7: Sequence grammar that drives phase separation of unfolded states is similar between barnase and disease associated IFPs.

Figure 7:

(A) Fraction of total mean contact probability per residue type calculated from atomistic simulations of the unfolded state (STAR Methods). (B) Fluorescence micrographs show deposits formed by a destabilized variant of barnase (I25A, I96G) flanked with fluorescent proteins (mTFP1 and Venus) (Wood et al., 2018) along with deposits formed by mutant SOD1 (SOD1 A4V) or mutant Httex1 containing a glutamine tract of 72 residues fused to mCherry. The constructs were co-transfected in HEK293T cells. Outlines of cells and nuclei are shown with dashed lines.