Table 2.

Specific primers for PCR sequencing of HBB gene.

HBB gene	Specific primer pairs	Annealing Tm	PCR product sizes
Exon 1 and 2	F: 5′ CTGAGGGTTTGAAGTCCAACTCC 3′	60°C	808 bp
	R: 5′ CTTCCACACTGATGCAATCATTC 3′
Exon 3	F: 5′ CAATGTATCATGCCTCTTTGCAC 3′	60°C	580 bp
	R: 5′ GCACTGACCTCCCACATTCC 3′