FIG. 4.

Presence of Vag8 in other Bordetella species. Antiserum which reacted with the maltose binding protein–Vag8 fusion was raised in mice and used in immunoblots of whole-cell lysates of Bordetella species grown on BG plates. Each lane represents approximately 2 × 10⁸ cells. (A) Lane 1, B. pertussis 18323; lane 2, B. bronchiseptica 058; lane 3, B. bronchiseptica 110H; lane 4, B. bronchiseptica 207. (B) Lane 1, B. parapertussis 13367; lane 2, B. parapertussis 9807; lane 3, B. pertussis 18323. The arrows indicate Vag8. The migrations of the molecular size markers are indicated.