Figure 6.

TGF-β₁increases interaction of Sp1 and KLF6 on prolidase promoter: NIH3T3 cells plated in 6-well plates were serum-starved for 6 h were stimulated further with TGF-β1 for 6 h. Following treatment, the cells were subjected to ChIP analyses to probe the binding of Sp1 and KLF6 to the PEPD promoter using anti-KLF6, anti-Sp1 and IgG (control) antibodies. Co-precipitated chromatin fragments were purified and analyzed by PCR amplification using primers (Table 1) against the mouse PEPD proximal promoter region. PCR products were separated by agarose gel electrophoresis. Results were normalized to total input chromatin. A, representative blot and (B) Densitometry analyses of PCR amplified DNA from ChIP assay. Data in (B) are mean values of three independent experiments with error bars representing SEM. ∗ p value of < 0.05 for the statistical comparison of untreated vs TGF-β1 treated samples.