Figure 2.

Growth curves of MR-22 against E. coli ATCC 25922 (A) and E19 (B). Killing kinetics with E. coli ATCC 25922 (C) and E19 (D). Different concentrations of MR-22 were incubated with approximately 1x10⁶ CFU/ml inoculum. Samples were taken at different time intervals, plated in LB medium and CFUs counted by triplicate. (E) Cytotoxicity of MR-22 against L-O2 cells and HK-2 cells determined by CCK-8 assay. (F) Hemolytic activities of MR-22 at different concentrations after incubation with 4% human red blood cells for 1 h. Data are presented as the mean ± standard deviation of three independent experiments. ^**** P< 0.0001 compared to the positive control (PC) group. The error bars indicate the mean ± standard deviation (n = 3).