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Proliferative responses of T lymphocyte-enriched splenocyte cultures from B. melitensis 16M-infected mice at 28 weeks postinfection. Lymphocytes were stimulated with antigen (1-μg protein concentration) for 96 h and pulsed with [3H]thymidine for 18 h. Antigens: RSET, E. coli BL21 (DE3)/pRSETB cell lysate; RSET-BA14K, E. coli BL21 (DE3)/pRS44.8; 2308, B. abortus 2308 whole killed cells. Stimulation indices were calculated as described in the text. ∗, P < 0.05 compared to RSET.
