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. 2024 Feb 6;15:974. doi: 10.1038/s41467-024-44905-z

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — agenT-797 is manufactured by isolation of iNKT cells from peripheral blood mononuclear cells of healthy donors, iNKT-specific stimulation and expansion in vitro. A Levels of circulating iNKT cells were measured in PBMCs of 15 healthy donors and manufacturing cut-off was set at 0.05% of CD3+ lymphocytes (filled, above cut-off, open, below cut-off). B From selected donors, iNKT cells were purified and cells expanded using MiNK Therapeutics’ manufacturing protocol. At the end of manufacturing, purity of culture measured >99%. C Cytokines that were produced during expansion of iNKT cells were measured from supernatant cultures at the end of manufacturing. Both T_h1 (IFN-γ, GM-CSF, TNF-α) and T_h2 (IL-4, IL-5, IL-13) cytokines were produced. Data from two separate manufacturing runs using starting material from different donors (bars represent mean). agenT-797 iNKT cell products were subjected to flow cytometric analysis of intracellular cytokines (D) and surface markers (E) as shown. The CD4+ and negative iNKT populations expressed distinct levels of each marker. Data representative of agenT-797 batches manufactured to date (>10).