Fig. 2. Post-infusion changes in serum biomarkers.

Serum cytokine levels broken into (A) CRS markers and (B) functional classes from pre- and post-infusion timepoints indicated. Pre-infusion serum sample was taken on day of infusion with agenT-797 (ID0 Pre). Cytokines were measured by Luminex using a custom multiplex assay (ThermoFisher). A Serum levels of key indicators of CRS; IL-1α, IL-1β, IL-6, ferritin, C-reactive protein (CRP) and D-dimer levels. Biomarker concentration determined over 28 days. B Serum cytokine levels of selected biomarkers spanning the immuno-regulatory spectrum. Cytokine data is binned as described below. Dotted lines indicate upper limit normal level of respective biomarker in healthy people. Cytokine data are grouped into pre-infusion sample (pre, single timepoint on day 1), early post-infusion window (average from 2 h post infusion on day 1 to day 7 post-infusion; D1-7) corresponding to the measured persistence of agenT-797 in the periphery, and a late post-infusion time window (days 10–28; D10-28). Data for ferritin, CRP, D-dimer shown for pre-infusion timepoint on day of treatment (pre, singe timepoint), early post infusion window (days 2–8; D2-8) and late post-infusion time window (days 8–28; D8-28). IL-1α/β not detected or within normal range. Cytokine data from all patients (n = 20), except for IL-4, IL-13, IL-15, IP-10 and VEGF-D where data are available only for cohorts 1 and 2 (n = 7). Data for ferritin, CRP, D-dimer measurements from 11 patients (Cohort 1: n = 3; Cohort 2: n = 2; Cohort 3: n = 6). All sample measurements returned as under the limit of detection scored as zero. All significant changes (adjusted p value < 0.05) post dosing are indicated (column means comparison using ANOVA/mixed effect model followed by Tukey’s multiple comparisons test). Box plot whiskers extends to the minimum and maximum values, with the box encompassing the interquartile range (IQR), and the center line indicating the median.