Fig. 2. Identification of broadly neutralizing antibodies against severe human coronaviruses.

a Schematic representation of the isolation process for potential broadly neutralizing antibodies using a combined method of single B-cell sorting and virtual screening. b Heatmap showing the binding EC₅₀ values of the selected mAbs with S trimers of MERS-CoV, SARS-CoV, and SARS-CoV-2, respectively. c Heatmap showing the neutralization IC₅₀ values of each mAb with indicated pseudotyped viruses. d Neutralization curves (left) and IC₅₀ values (right) of indicated mAb with MERS-CoV, SARS-CoV, and SARS-CoV-2 pseudotyped viruses, respectively. The data are representative of one of at least three independent experiments and are presented as the mean ± SEM.