Figure 5.

In vivo functional response of D1R-SPNs to D1R activation in Tg(Drd1a-Cre); FLIM-AKAR^flox/flox mice. (a) Schematic of experimental setup for fluorescence lifetime photometry (FLiP). (b) Example trace of change in fluorescence lifetime in response to saline followed by SKF 81297 (10 mg/kg) in D1R-SPNs in the dorsal striatum. (c) Quantitation of change of fluorescence lifetime in response to saline or SKF 81297 (n = 5 animals). Data represent maximum change in lifetime after intraperitoneal injections of saline or SKF 81297 (*p < 0.05, one-tailed Wilcoxon signed rank test).