Fig. 4.

Differential expression analysis of Treg functional markers and chemokine receptors comparing healthy controls (n = 32), people with mild (n = 15) and moderate-severe psoriasis (n = 16). (a) UMAP plots of CD4+ Tregs aggregated across all samples showing marker expression (arcsinh-transformed intensity) of CCR4 and CCR7 within each cohort. (b) Heatmap of z-scored normalized expression across all samples of markers on Tregs and CLAhi Tregs with markers organized by hierarchical cosine distance clustering. Red heatmap values represent relatively higher marker expression and blue heatmap values represent relatively lower marker expression. (c) Overlaid violin and swarm plots of CCR4, CCR7 and FoxP3 on Tregs and CLAhi Tregs comparing healthy control, people with mild and moderate-severe psoriasis. (d) Integrated heatmap dot plot showing pairwise comparisons of cohorts with the log2 fold change and adjusted p-values of Treg markers on Tregs and CLAhi Tregs. An asterisk indicates a significantly differentially expressed marker with an adjusted p < 0.05. ∗adj. p < 0.05, ∗∗adj. p < 0.01, ∗∗∗adj. p < 0.001, ∗∗∗∗adj. p < 0.0001.