FIG. 1.

PCR-based serotyping of strain DAW. capH- to capK-containing DNA fragments from serotype 5 strain 8325-4 (lanes 2, 5, and 8), serotype 8 strain Becker (lanes 4, 7, and 10), and the methicillin-resistant clinical isolate DAW (lanes 3, 6, and 9) were electrophoresed in a 0.8% agarose gel. The PCR fragments were digested with the restriction endonuclease Tth111I (lanes 5 to 7) or SmaI (lanes 8 to 10) or were not treated with a restriction endonuclease (lanes 2 to 4). Lane 1 contains the Supermarker (Bioworks) DNA size standard.