Carbohydrate profiles of B. subtilis determined by GC-MS. (A) Cells grown in phosphate-rich medium. (B) Cells grown in phosphate-limited medium. Note that the level of anhydroribitol (marker for teichoic acid) is markedly reduced under phosphate-limited growth conditions. Other peaks: 2, ribose plus ribitol; 3, arabinitol (internal standard); 4, glucose; 5, muramic acid; 6, methylglucamine (internal standard); 7, glucosamine.