REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat-anti human IgG-Fc	Bethyl Laboratories	A80-104A
MSD SULFO-TAG Labeled Anti-Human Antibody (Goat)	MSD	R32AJ-1
Anti-human CD45 PerCP/Cy5.5	BioLegend	304028
Anti-mouse CD45 Dazzle594	BioLegend	103146
Anti-human CD3 BV650	BioLegend	317324
Anti-human CD4 AF700	BioLegend	357418
Anti-human CD8 BV785	BioLegend	344740
Anti-human CD19 BV421	BioLegend	302234
Anti-human CD27 BV711	BioLegend	356430
Anti-human CD62L BV510	BioLegend	304844
Anti-human PD1 PE	BD	557946
Anti-human TIM-3 APC/Cyanine7	BioLegend	345026
Anti-human CD45RA PerCP/Cyanine5.5	BioLegend	304122
Anti-human CD45RO PE/Cyanine7	BioLegend	304230
Bacterial and virus strains
pHAGE-Ef1a-IRES-WPRE	This paper	N/A
Biological samples
Leukopak	StemCell	70500
Apheresis leukoreduction collars	obtained from the blood bank of the Brigham and Women's Hospital under DFCI approved IRB protocol #14-343	N/A
Chemicals, peptides, and recombinant proteins
Polyethylenimine, PEI
Lenti-X™ Concentrator	Clontech	631231
Ficoll-Paque-PLUS	GE Healthcare	45001750
Gentamicin	Gibco	15750060
IL-21	Miltenyi Biotec	130-095-784
IL-7	Miltenyi Biotec	130-095-363
IL-15	Miltenyi Biotec	130-095-765
D-luciferin potassium salt	Promega	E1605
Critical commercial assays
Zombie Yellow™ Fixable Viability Kit	BioLegend	423104
Tumor Dissociation Kit	Miltenyi Biotec	130-095-929
CD34 microbead kit	Miltenyi Biotec	130-100-453
CD4 microbeads	Miltenyi Biotec	130-097-048
CD8 microbeads	Miltenyi Biotec	130-097-057
TransAct kit	Miltenyi Biotec	130-111-160
Deposited data
scRNAseq data	This paper	GSE210664
Kevin Bi et al.	Single Cell Portal	Single Cell Portal: https://singlecell.broadinstitute.org/single_cell/study/SCP1288/tumor-and-immune-reprogramming-during-immunotherapy-in-advanced-renal-cell-carcinoma#study-summary
Codes	This paper	https://doi.org/10.5281/zenodo.7274663
Supplementary tables	This paper; Zenodo Data	Table S2∼10; https://doi.org/10.5281/zenodo.10456704
Software and algorithms
Cellranger	10x genomics	https://www.10xgenomics.com/support/software/cell-ranger
Seurat	Hao et al.99	https://satijalab.org/seurat/
harmony	Korsunsky et al.100	https://portals.broadinstitute.org/harmony/index.html
CellChat	Jin et al.64	https://github.com/sqjin/CellChat
Velocyto	La Manno et al.50	https://velocyto.org/