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. 2024 Feb 6;12(2):e2392. doi: 10.1002/mgg3.2392

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© 2024 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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The result of the multiplex PCR on a 2% agarose gel. (a) Multiplex A. Lane 1: Molecular weight marker (72–1353 bp) phi X‐HeaIII; Lane 2: Negative control (water); Lane 3: The female DNA control, Lanes 4 and 6: Healthy men DNA samples without microdeletion; Lane 5: Men with couples experiencing RPL (microdeletion for sY134‐AZFb). (b) Multiplex B. Lane 1: Molecular weight marker (72–1353 bp) phi X‐HeaIII; Lane 2: Negative control (Water); Lane 3: The female DNA control; Lane 4: Healthy men DNA sample (without microdeletion); Lane 5: Men with couples experiencing RPL (microdeletion for sY127‐AZFb); Lane 6: Men with couples experiencing RPL (microdeletion for sY254‐AZFc).