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Model for interfacial assembly of OhyA on the membrane bilayer. OhyA is a peripheral membrane protein that is anchored to the membrane bilayer through its carboxy terminus (blue). OhyA extracts oleate (18:1) from the bilayer, then binds flavin adenine dinucleotide (FAD) for catalysis. OhyA catalyzes water addition to 18:1 to produce 10-hydroxystearic acid (h18:0), and then releases FAD into the cytosol and h18:0 into the membrane.
