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. 2024 Jan 10;300(2):105627. doi: 10.1016/j.jbc.2024.105627

Figure 4.

Figure 4

Functional characterization of the OhyA carboxy terminus.A, cryo-electron microscopy (cryo-EM) particle patch from a micrograph of OhyA bound to a POPG:POPC liposome. The scale bar corresponds to 10 nm. B, cryo-EM 2D class average (representing 5374 particles) of OhyA association with POPG:POPC liposomes. The scale bar corresponds to 10 nm. C, surface plasmon resonance determination (n = 3 technical replicates) of DOPG/DOPC liposome binding affinity of OhyA (black) and OhyA(ΔHTH) (blue). The data from three independent titrations were fit to the Hill equation to calculate the KD. Mean ± S.D. D, specific activities (n = 3 technical replicates) of OhyA (13.887 ± 0.343 pmol/min/μg) and OhyA(ΔHTH) (0.0003 ± 0.00005 pmol/min/μg). Unpaired t test determined if differences between OhyA and OhyA(ΔHTH) specific activities have statistical significance and determined the two-tailed p value. Mean ± S.D.