Figure 9.
OhyA CTD peptide(550–591) interacts with DOPG liposomes.A, 1D 1H-spectra of 1 mM OhyA CTD peptide(550–591) in 150 mM NaCl, with increasing concentration of DOPG liposomes as indicated. The line broadening of resonances indicate OhyA CTD peptide(550–591) binds DOPG liposome. B, line broadening of resonances quantified as B (34), plotted as a function of concentration. B is an intensity ratio derived from one-(IB/IF) where IB and IF are the intensities of the peaks from the peptide in liposome-bound and liposome-free forms respectively. Dots represent experimental values and lines the fitted equation to derive the KD values. C, quantitative analysis of the normalized intensity of the peaks from (A) and Fig. S13 plotted as a function of the residue number. The same coloring scheme in the spectra is used.