Table 1.
Cryo-EM data collection, model composition, refinement, and validation parameters
| Structure | OhyA dimer (ordered CTD) | OhyA dimer (disordered CTD) |
|---|---|---|
| EMDB ID | EMD-42480 | EMD-42484 |
| PDB ID | 8UR3 | 8UR6 |
| Data collection | ||
| Microscope | Talos Arctica (FEI) | Talos Arctica (FEI) |
| Voltage (kV) | 200 | 200 |
| Detector | K3 BioQuantum (Gatan) | K3 BioQuantum (Gatan) |
| Pixel size (Å) | 1.044 | 1.044 |
| Defocus range (μM) | −0.5 to −2.0 | −0.5 to −2.0 |
| Movies | 3246 | 3246 |
| Frames/movie | 50 | 50 |
| Total dose (electrons/Å2) | 50 | 50 |
| Model composition | ||
| Chains | 2 | 2 |
| Non-hydrogen atoms | 9376 | 7540 |
| Protein residues | 1158 | 930 |
| Refinement | ||
| Number of particles | 572,989 | |
| Resolution (Å) | 2.61 | 3.03 |
| RMS deviations | ||
| Bond length (Å) (# > 4σ) | 0.003 (0) | 0.007 (0) |
| Bond angles (°) (# > 4σ) | 0.511 (0) | 0.760 (0) |
| Validation | ||
| MolProbity score | 1.32 | 1.22 |
| Clash score | 4.05 | 4.39 |
| Ramachandran plot (%) | ||
| Outliers | 0.00 | 0.00 |
| Allowed | 2.7 | 1.88 |
| Favored | 97.3 | 98.12 |
| Rotamers (%) | ||
| Outliers | 0.0 | 0.0 |
| Favored | 99.7 | 99.6 |
| Cβ outliers (%) | 0 | 0 |
| Peptide plane (%) | ||
| Cis proline/general | 0.0/0.2 | 0.0/0.0 |
| Twisted proline/general | 0/0 | 0/0 |