TABLE 1.
Correlation of CDF activity with HA/P production by V. cholerae
| Strain | Description (reference) | CDF activitya | HA/P levelsb |
|---|---|---|---|
| O395 | Classical, India (21) | 0.5 | − |
| RV508 | Classical, derivative of 569B (21) | 1 | − |
| 2740-80 | El Tor, U.S. Gulf Coast, CTXφ− (23) | 666 | ++++ |
| CO945 | El Tor, India (26) | 9 | ++ |
| C6709 | El Tor, Peru (28) | 55 | +++ |
| P27459 | El Tor, Bangladesh (21) | 38 | +++ |
| Bang 15 | El Tor, CTXφ− nonmotile derivative of P27459 (23) | 1,408 | ++++ |
| MO45 | O139, India (29) | 0.6 | − |
| MO10 | O139, India (29) | 0.8 | + |
| Bengal 15 | O139, CTXφ− nonmotile derivative of MO10 | 0.7 | + |
| 3083 | El Tor, Vietnam | 52 | +++ |
| HAP-1 | 3083 derivative, hap::kan (12) | 1 | − |
| HAP-1(pCH2) | 200 | NDc |
a
CDF activities (arbitrary units) were determined by a bioassay as described in Materials and Methods.
b
Total protein was precipitated from 1-ml portions of 16-h culture supernatants by addition of 10% TCA. SDS-solubilized protein was fractionated by SDS-PAGE, and HA/P levels were determined semiquantitatively by Western blot analysis with rabbit anti-HA/P antiserum, as described in Materials and Methods.
c
ND, not determined.