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. 2024 Jan 22;70:103054. doi: 10.1016/j.redox.2024.103054

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© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 1 — Ldlr^−/−mice transplanted with Acod1^−/−bone marrow contain more circulating and splenic Ly6c^Lowpatrolling monocytes. A) Irradiated atherosclerosis-susceptible Ldlr^−/− mice were transplanted with either Acod1^+/+ or Acod1^−/− bone marrow (BM) and were given 6 weeks for efficient engraftment prior to initiating a high-fat diet (HFD). Blood samples are taken on weeks 0, 5 and 8 of HFD and mice were sacrificed on week 9. B) leukocyte subsets in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) mice at week 8 expressed as a percentage of circulating CD45⁺ cells (n = 22/23 for Acod1^+/+ → Ldlr^−/−/Acod1^−/− → Ldlr^−/−, unpaired t-test). C) Blood monocytes in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) mice at week 0, 5 and 8 expressed as a percentage of circulating CD45⁺ cells (n = 22/23 for Acod1^+/+ → Ldlr^−/−/Acod1^−/− → Ldlr^−/−, two-way ANOVA). D) Blood Ly6C monocyte subsets in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) mice at week 8 expressed as a percentage of circulating CD45⁺ cells (n = 22/23, two-way ANOVA). E) Splenic monocytes in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) BM expressed as a percentage of splenic CD45⁺ cells (n = 17/18, unpaired t-test). F) Splenic Ly6C monocyte subsets in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) mice expressed as a percentage of splenic CD45⁺ cells (n = 17/18, two-way ANOVA). G) Ly6C low (left), intermediate (middle) and high (right) counts per mL of blood in Acod1^+/+ → Ldlr^−/− (filled) or Acod1^−/− → Ldlr^−/− (checkered) mice at weeks 0, 5 and 8 of HFD (n = 22/23, two-way ANOVA). Gating strategies for flow cytometry is provided in Supplemental Fig. 3. Data points display individual mice and error bars show SD.