TABLE 1.
Day postinfection | Mean IFN-γ concn (pg/ml) ± SEMa
|
|
---|---|---|
i.v. | i.n. | |
7 | 225.50 ± 12.1 | 203.58 ± 68.1 |
14 | 330.70 ± 92.1 | 121.90 ± 9.3 |
21 | 235.80 ± 6.4 | 9.10 ± 9.8 |
28 | 25.64 ± 5.4 | 4.56 ± 1.20 |
Nylon wool-purified genital T cells were isolated at the indicated time points from mice infected by different routes. The cells were stimulated with chlamydial antigen for 5 days, and the amounts of IFN-γ in the culture supernatants were measured by ELISA as described in Materials and Methods. No IFN-γ was detected in samples from p.o.- and s.c.-immunized mice.