TABLE 2.
IFN-γ secreted by MoPn-specific T cells from genital tracts of BALB/c mice preinfected with MoPn by different routes and then challenged i.v.
| Time (h) postchal- lenge | Mean IFN-γ concn (pg/ml) ± SEMa
|
|||
|---|---|---|---|---|
| p.o. | i.v. | i.n. | s.c. | |
| 24 | 0.0 ± 0.0 | 25.50 ± 6.2 | 88.50 ± 6.1 | 0.0 ± 0.0 |
| 48 | 6.0 ± 2.0 | 50.20 ± 18.1 | 160.21 ± 9.3 | 2.0 ± 0.40 |
| 72 | 14.0 ± 2.0 | 89.80 ± 16.4 | 364.12 ± 58.8 | 10.0 ± 2.0 |
| 144 | 196.0 ± 23.0 | 125.64 ± 25.4 | 256.6 ± 18.20 | 26.0 ± 3.0 |
| 288 | 140.0 ± 20.6 | 288.74 ± 22.0 | 240.46 ± 26.0 | 134.0 ± 21.6 |
| 360 | 196.0 ± 23.0 | 322.24 ± 32.0 | 202.24 ± 42.5 | 296.0 ± 15.0 |
a
Nylon wool-purified genital T cells were isolated at the indicated time points from mice infected by different routes. The cells were stimulated with chlamydial antigen for 5 days, and the amounts of IFN-γ in the culture supernatants were measured by ELISA as described in Materials and Methods.