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. 2024 Jan 24;15:1345236. doi: 10.3389/fmicb.2024.1345236

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Copyright © 2024 Fan, Zhang, Wang, Miao, Zhang, Jiang, Qi, Zhang, Hui, Zhang, Yue, Zhou, Li, Wang, Chen and Hu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant virus identification. Panels (A–E) are PCR identification and purification results of SY18ΔL7L, SY18ΔL8L, SY18ΔL9R, SY18ΔL10L, SY18ΔL11L, panels (F–J) are the fluorescence maps of each single-gene deletion viruses, and panels (K–O) are the bright field of each single-gene deletion viruses. M: DL5000 marker; a1~a3, b1~b3, c1~c3, d1~d3, e1~e3: each single-gene deletion virus detection samples; 4: ASFV SY18 control.SY18ΔL7L and SY18ΔL8L amplified a 643 bp fragment in the presence of ASFV SY18; SY18ΔL9R amplified a 280 bp fragment in the presence of ASFV SY18; SY18ΔL10L, and SY18ΔL11L amplified a 200 bp fragment in the presence of ASFV SY18.