FIG. 3.

The tyrosine kinase inhibitor erbstatin blocks DAEC C1845-induced F-actin ruffles and F-actin stress fiber disappearance in infected postconfluent cultured human embryonic intestinal INT407 cells. After bacterial adhesion (3 h at 37°C), F-actin filaments were localized with FITC-phalloidin. Cells were treated prior to infection with erbstatin (1 h, 150 μM), and the inhibitor remained present in the incubation medium during the bacterial infection. (A) Uninfected control cells. (B) DAEC C1845-infected cells. (C) Uninfected control cells treated with erbstatin. (D) DAEC C1845-infected cells treated with erbstatin.