FIG. 5.

Dose-response effects of a calcium inhibitor or chelator on DAEC C1845-induced F-actin rearrangements in infected postconfluent cultured human embryonic intestinal INT407 cells. Cells were treated prior to infection with the calcium inhibitor dantrolene (0.5 h) or the calcium chelator BAPTA/AM (1 h). Drugs remained present in the incubation medium during the bacterial infection. After bacterial adhesion (3 h at 37°C), F-actin filaments were localized with FITC-phalloidin. F-actin alterations were scored as described by Kotani et al. (18). (A) Uninfected control cells. (B) DAEC C1845-infected cells. (C) DAEC C1845-infected cells treated with BAPTA/AM (25 μg/ml).