FIG. 6.

The PI 3-kinase inhibitor wortmannin blocks DAEC C1845-induced F-actin rearrangements in infected postconfluent cultured human embryonic intestinal INT407 cells dose dependently. Cells were treated prior to infection with wortmannin for 0.75 h. The inhibitor remained present in the incubation medium during the bacterial infection. After bacterial adhesion (3 h at 37°C), F-actin filaments were localized with FITC-phalloidin. F-actin alterations were scored as described by Kotani et al. (18).