KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| APC/eFluor 780 anti-mouse CD45 rat monoclonal | eBioscience | Cat# 47-0451-82; RRID: AB_1548781 (Clone 30-F11) |
| Alexa Fluor 700 anti-mouse CD11b rat monoclonal | eBioscience | Cat# 56-0112-82; RRID: AB_657585 (Clone M1/70) |
| eFluor 450 anti-mouse F4/80 rat monoclonal | eBioscience | Cat# 48-4801-82; RRID: AB_1548747 (Clone BM8) |
| PE/Cy7 anti-mouse Ly6G rat monoclonal (clone 1A8) | Biolegend | Cat# 127618; RRID: AB_1877261 |
| APC anti-mouse Ly6C rat monoclonal (clone HK1.4) | eBioscience | Cat# 17-5932; RRID: AB_1724155 |
| Alexa Fluor 488 anti-mouse CD206 rat monoclonal | Biolegend | Cat# 141710; RRID: AB_10900445 (clone C068C2) |
| PE anti-mouse MHCII rat monoclonal | eBioscience | Cat# 12-5321-82; AB_465928 (clone M5/114.15.2) |
| APC anti-mouse EpCam rat monoclonal (clone G8.8) | BD Biosciences | Cat# 563478; RRID: AB_2738234 |
| PerCp/Cy5.5 anti-mouse CD64 rat monoclonal | Biolegend | Cat# 139308; RRID: AB_2561963 (clone X54-5/7.1) |
| FITC anti-mouse CD3e Armenian hamster | eBioscience | Cat# 11-0031-82; RRID: AB_464882 monoclonal (clone 145-2C11) |
| PerCp anti-mouse CD4 rat monoclonal (clone GK1.5) | Biolegend | Cat# 100434; RRID: AB_893324 |
| APC anti-mouse CD8a rat monoclonal (clone 53-6.7) | eBioscience | Cat# 17-0081-83; RRID: AB_469336 |
| PE anti-mouse gd-TCR Armenian hamster | BD Biosciences | Cat# 553178; RRID: AB_394689 monoclonal (clone GL3) |
| Alexa Fluor 700 anti-mouse CD29 Armenian hamster | Biolegend | Cat# 102218; RRID: AB_493711 monoclonal (clone HMbeta1-1) |
| Brilliant Violet 421 antimouse CD34 rat monoclonal | Biolegend | Cat# 119321; RRID: AB_10900980 (clone MEC14.7) |
| APC-Fire750 anti-mouse CD31 rat monoclonal (390) | Biolegend | Cat# 102434; RRID: AB_2629683 |
| Anti-CD31 (PECAM-1) Armenian hamster monoclonal | Millipore | Cat# MAB1398Z, RRID:AB_94207 (clone 2H8) |
| Rat Anti-Mouse CD31, Clone MEC 13.3 (RUO) | BD Biosciences | Cat# 550274, RRID: AB_393571 |
| Anti-ER-TR7 rat monoclonal | Abcam | Cat# ab51824; RRID: AB_881651 |
| Anti-GFP chicken polyclonal | Abcam | Cat# ab13970; RRID: AB_300798 |
| Anti-mouse/human CD207 (Langerin) Antibody | BioLegend | Cat# 144202; RRID: AB_2562088 |
| Rabbit Anti-mouse Lyve1 antibody | Abcam | Cat# ab14917; RRID: AB_301509 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma | T5648 |
| EdU (5-ethynyl-2’-deoxyuridine) | Invitrogen | E10187 |
| Sytox Orange | Invitrogen | S34861 |
| Sytox Blue | Invitrogen | S34857 |
| Collagenase 1 | Worthington | LS004196 |
| Liberase TM | Roche | 5401127001 |
| Liberase TL | Roche | 5401020001 |
| Ethyl cinnamate | Sigma-Aldrich | 112372 |
| Critical Commercial Assays | ||
| Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit | Invitrogen | C10419 |
| Click-iT EdU Alexa Fluor 647 Imaging Kit | Invitrogen | C10340 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | Charles River Laboratories | 027 |
| Mouse: B6.FVB-Tg(CD207-Dta)312Dhka/J | The Jackson Laboratory | 017949 |
| Mouse: Tg(CD207-cre/ERT2)1Dhka/J | The Jackson Laboratory | 028287 |
| Mouse: B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | The Jackson Laboratory | 007676 |
| Deposited Data | ||
| Haensel et al. (2020) scRNA-seq dataset (raw) | GEO | GSE142471 |
| Konieczny et al. (2022) scRNA-seq dataset (processed) | GEO | GSE166950 |
| Analysis Code to reproduce analysis | Github depository | https://github.com/khbridges/waskolangerhans DOI: 10.5281/zenodo.7324227 |
| Software and Algorithms | ||
| FIJI (ImageJ) | NIH | https://fiji.sc |
| Adobe Photoshop | Adobe | https://www.adobe.com/products/photoshop.html |
| FlowJo | FlowJo, LLC | https://www.flowjo.com |
| GraphPad Prism | GraphPad Software, Inc | https://www.graphpad.com |
| Scanpy | Python | https://github.com/theislab/scanpy |
| Tensorflow | Python | https://www.tensorflow.org |
| NicheNet | R | https://github.com/saeyslab/nichenetr |
| Circlize (for chord diagram visualization) | R | https://github.com/jokergoo/circlize |
| Seurat 3.0 | Stuart et al., 2019 | https://satijalab.org/seurat/ |
| Cell Ranger Single-Cell Software Suite | 10x Genomics | https://support.10xgenomics.com/singlecell-gene-expression/software/pipelines/latest/what-is-cell-ranger |