The 3D surface is first mapped via to the plane, either through Ricci flow
(which is slower but results in a more exactly conformal map) or through
minimization of a Dirichlet energy (faster but less precisely conformal, see
Supplementary
Information Section VIIa). In either case, the material is
periodic in the dimension and finite in extent along the
longitudinal direction . The resulting coordinate system is then
adjusted. First we apply , where is a distance along the longitude of the
tissue defined by Eq. (1),
which we find aids in parameterization for tubes with varying radii (Supplementary Information
Section VIIb). If the timepoint under question is the reference
timepoint , this defines the material coordinates.
Otherwise, if , we then apply , where is given by Eq. (2), and then apply
to stabilize the resulting coordinates
based on material motion measured through particle image velocimetry (phase
correlation analysis) relative to the previous timepoint.