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. Author manuscript; available in PMC: 2024 Jun 6.
Published in final edited form as: Nat Methods. 2023 Dec 6;20(12):1980–1988. doi: 10.1038/s41592-023-02081-w

FIG. Extended Data Fig. 2. Overlaid pullback images spanning morphogenesis demonstrate the stability of the pullback parameterization against 3D motion of the tissue.

FIG. Extended Data Fig. 2.

Using planar maps of the folding Drosophila midgut, we perform refined Lagrangian parameterization of the surface. The resulting timepoints at 0 minutes, 35 minutes, and 70 minutes after constriction onset are overlaid in cyan, magenta, and yellow, respectively. Much of the tissue appears as black and white, indicating that tissue placement in the pullback frame is stationary.