Table 1.
Antibody and reagent list.
| Vendor | Cat no. | Reagent | Notes |
|---|---|---|---|
| BD Biosciences | 553142 | BD Pharmigen: Rat antimouse CD16/CD32 | Clone: 2.4G2 |
| BD Biosciences | 567316 | BD Pharmigen: APC Rat antimouse CD25 | Clone: BC96 |
| BD Biosciences | 552772 | Bd Pharmigen: PE-Cy7 Rat antimouse B220 | Clone: RA3-6B2 |
| BD Biosciences | 553133 | BD Pharmigen: FITC antimouse CD44 | Clone: IM7 |
| BD Biosciences | 558448 | BD Phosflow: PE mouse anti-CD247 (pY142) | Clone: K25-40769 |
| BD Biosciences | 558577 | BD Phosflow: Alexafluor 647 mouse anti-Lck (pY505) | Clone: 4/LCK-Y505 |
| Biolegend | 933102 | PE mouse anti-Lck (pY394) | Clone: A18002D |
| BD Biosciences | 557818 | BD Phosflow: Alexafluor 488 mouse anti-ZAP70 (pY319)/Syk (pY352) | Clone: 17A/P-ZAP70 |
| BD Biosciences | 558519 | BD Phosflow: Alexafluor 488 mouse anti-LAT (pY171) | Clone: I58-1169 |
| BD Biosciences | 558438 | BD Phosflow: Alexafluor 647 mouse anti-SLP76 (pY128) | Clone: J141-668.36.58 |
| Cell Signaling Technology | 14461S | PE rabbit anti-PLCγ1 (pY83) | D6M9S |
| BD Biosciences | 558275 | BD Phosflow: PE anti-Akt (pT308) | J1-223.371 |
| BD Biosciences | 561670 | BD Phosflow: Alexafluor 647 mouse anti-Akt (pS473) | Clone: M89-61 |
| Biolegend | 300314 | LEAF Purified antihuman CD3 | Clone: HIT3a |
| Biolegend | 302914 | LEAF Purified antihuman CD28 | Clone: CD28.2 |
| Biolegend | 102104 | Biotin-antimouse CD28 | Clone: 37.51 |
| Biolegend | 100244 | Biotin-antimouse CD3 | Clone: 17A2 |
| Biolegend | 405151 | Purified Steptavidin | — |
| Cell Signaling Technology | 5861S | Rabbit anti-NFAT1 XP | Clone: D43B1 |
| Cell Signaling Technology | 8242S | Rabbit anti-NF-kappa B p65 XP | Clone: D14E12 |
| Cell Signaling Technology | 2250S | Rabbit anti-cFos | Clone: 9F6 |
| eBioscience | 17-7311-81 | APC antimouse IFNγ | Clone: XMG1.1 |
| Invitrogen | 16-0031-86 | Antimouse CD3e | Clone: 145-2C11 |
| Invitrogen | 16-0281-86 | Antimouse CD28 | Clone: 37.51 |
| Invitrogen | 31115 | Goat anti-hamster IgG (H + L) | |
| Invitrogen | A11011 | Alexafluor 568 goat antirabbit IgG (H + L) | |
| Invitrogen | A21451 | Alexafluor 647 goat anti-hamster IgG (H + L) | |
| Invitrogen | P36935 | Prolong Gold antifade reagent with DAPI | |
| Invitrogen | A12379 | Alexa Fluor 488 phalloidin |