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. 2024 Feb 7;20:49. doi: 10.1186/s12917-024-03890-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — Construction and activity verification of TCONS_00007391 RNA interference/overexpression vector. A Double enzyme digestion of the TCONS_00007391 interference recombination plasmid. M, Marker; 1, empty vector; 2–3, shRNA1; 4–5, shRNA2; 6–7, shRNA3. B Sanger sequencing of the TCONS_00007391 interference recombination plasmid. C Relative expression of TCONS_00007391 in macrophages transfected with shRNA vectors for 48 h as measured by RT-qPCR. D Bacterial liquid PCR amplification of TCONS_00007391. M, Marker; 1–6, TCONS_00007391. E Relative expression of TCONS_00007391 in macrophages transfected with overexpression vector for 48 h as measured by RT-qPCR