Fig 2.
Methylotrophic growth of Beijerinckiaceae bacterium RH AL1 with methanol as the carbon source (0.5%, vol/vol, 123 mM) and either different concentrations of La or different Ln elements. (A) Growth curves of strain RH AL1 grown with either two different concentrations (50 nM and 1 µM) of La or different Ln elements [1 µM of either La or Nd or 0.9 µM of a Ln cocktail (Ce, Nd, Dy, Ho, Er, Yb)]. Cultures without added Ln served as negative control. Cultivations were performed in triplicates (n = 3). Growth was monitored spectrophotometrically (OD600nm). The gray shade indicates the time interval considered for calculating doubling time (td) and growth rate (µ). Samples for molecular work were taken at time points t0–t2 including samples for RNA extraction (T1 ). (B) Growth monitoring by lanM gene-targeting quantitative PCR. Samples for DNA extraction and qPCR were taken at the beginning (T0 ), the mid- to late-exponential (T1 ), and stationary phase (T2 ).