TABLE 1.

Kinetics values of the hydrolysis of imipenem and cefepime by NDM-1 wild type and the K224R and K224I variants

	Imipenem			Cefepime			Taniborbactam
	k cat	K M	kcat/KM	k cat	K M	kcat/KM	IC50
	(s−1)	(µM）	(µM−1 s−1)	(s−1)	(µM）	(µM−1 s−1)	(µM)
NDM-1 (WT)	164 ± 5	28 ± 4	5.85 ± 0.85	82 ± 8	80 ± 5	1.02 ± 0.12	0.010 ± 0.001
NDM-1 (K224I)	184 ± 19	1644 ± 170	0.11 ± 0.01	90 ± 9	144 ± 15	0.63 ± 0.09	0.140 ± 0.014
NDM-1 (K224R)	73 ± 2	30 ± 4	2.43 ± 0.32	61 ± 6	750 ± 80	0.08 ± 0.01	NDa

^a ND, not determined.