TABLE 1.
Alterations in expression of selected QS-regulated genes by 100-µM Z-ethylthio enynonea
| Gene no. | Gene name | Pathogenesis factor | Function | Fold change | Adjusted P value |
|---|---|---|---|---|---|
| Downregulated QS virulence genes | |||||
| PA0051 | phzH | Phenazines | Oxidative stress | −5 | 9.28E-38 |
| PA0122 | rahU | Aegerolysins | Pore-forming toxin | −20 | 1.14E-75 |
| PA1148 | toxA | Toxic A | Cell death | −1 | 0.22 |
| PA0852 | cbpD | −33 | 3.93E-281 | ||
| PA1874 | − | Hypothetical protein (efflux) | Transport | −46 | 3.04E-71 |
| PA1899 | phzA2 | Phenazines | Oxidative stress | −39 | 5.15E-35 |
| PA1900 | phzB2 | Phenazines | Oxidative stress | −78 | 1.39E-139 |
| PA1901 | phzC2 | Phenazines | Oxidative stress | −46 | 3.04E-71 |
| PA1902 | phzD2 | Phenazines | Oxidative stress | −12 | 0.06 |
| PA1903 | phzE2 | Phenazines | Oxidative stress | −15 | 0.05 |
| PA1904 | phzF2 | Phenazines | Oxidative stress | −12 | 0.08 |
| PA1905 | phzG2 | Phenazines | Oxidative stress | -8 | 2.54E-12 |
| PA1871 | lasA | Protease | Host immune evasion | −32 | 2.86E-184 |
| PA2193 | hcnA | Hydrogen cyanide | Poor lung function | -4 | 7.20E-10 |
| PA2194 | hcnB | Hydrogen cyanide | Poor lung function | -4 | 5.81E-11 |
| PA2195 | hcnC | Hydrogen cyanide | Poor lung function | -4 | 1.78E-21 |
| PA2570 | lecA | Lectin A | Adhesion, cytotoxic | −156 | 4.19E-86 |
| PA3361 | lecB | Lectin B | Adhesion | −22 | 4.41E-75 |
| PA3478 | rhlB | Rhamnolipid | Immune evasion, motility | −15 | 4.00E-77 |
| PA3479 | rhlA | Rhamnolipid | Immune evasion, motility | −17 | 1.34E-58 |
| PA3692 | lptF | Lipotoxin F | Resistance to oxidative stress and adhesion | -8 | 2.20E-21 |
| PA3724 | lasB | Elastase | Iron acquisition | −76 | 7.97E-197 |
| PA4175 | piv | Endoprotease (protease IV) | Tissue damage | −39 | 5.15E-35 |
| PA4209 | phzM | Phenazines | Oxidative stress | −16 | 8.54E-13 |
| PA4210 | phzA1 | Phenazines | Oxidative stress | -6 | 1.08E-09 |
| PA4111 | phzB1 | Phenazines | Oxidative stress | −26 | 4.70E-92 |
| PA4212 | phzC1 | Phenazines | Oxidative stress | −20 | 1.14E-75 |
| PA4213 | phzD1 | Phenazines | Oxidative stress | -4 | 0.38 |
| PA4214 | phzE1 | Phenazines | Oxidative stress | -3 | 0.31 |
| PA4215 | phzF1 | Phenazines | Oxidative stress | -7 | 0.33 |
| PA4216 | phzG1 | Phenazines | Oxidative stress | -8 | 2.20E-21 |
| PA4217 | phzS | Phenazines | Oxidative stress | -4 | 3.00E-27 |
| − | hsiAH | T6SS (phospholipase D) | Cell lysis | Table S6 | |
| Upregulated QS virulence genes | |||||
| PA0996 | pqsA | Virulence and rhl regulator | 3 | 1.15E-08 | |
| PA0997 | pqsB | Virulence and rhl regulator | 3 | 5.11E-10 | |
| PA0998 | pqsC | Virulence and rhl regulator | 3 | 8.37E-10 | |
| PA0999 | pqsD | Virulence and rhl regulator | 3 | 7.96E-17 | |
| PA1000 | pqsE | Pseudomonas type III repressor gene C | 3 | 8.75E-105 | |
| Central QS regulators | |||||
| PA1430 | lasR | Transcriptional regulation | -3 | 1.53E-11 | |
| PA1431 | rsaL | Transcriptional regulation | -2 | 5.18E-04 | |
| PA1432 | lasI | Autoinducer synthesis | 1 | 0.55 | |
| PA3476 | rhlI | Autoinducer synthesis | -4 | 1.83E-45 | |
| PA3477 | rhlR | Transcriptional regulation | -3 | 2.39E-29 | |
a
All genes presented are categorized as being QS regulated by Hentzer et al. (22) The results are based on three independent experiments.