TABLE 1.
Bacterial strain | Source | Identifier/Reference |
---|---|---|
BW25993 | Yale Coli Genetic Stock Center | CGSC#: 7639 |
BW25993 ∆pspA | This study | N/A |
BW25993 hslU- TCtag | Bai Lab | (21) |
BW25993 hslU- TCtag ∆pspA | This study | N/A |
BW25993 hslU- egfp | This study | N/A |
BW25993 hslU- egfp ∆pspA | This study | N/A |
BW25993 ∆pspA with pBAD | This study | N/A |
BW25993 ∆pspA with pBAD-pspA | This study | N/A |
Plasmid | Description | Source |
pSIM6 | helper plasmid for homologous recombination, Ampicillin | (52, 53) |
pCP20 | helper plasmid for Flp recombinase-mediated excision, Ampicillin/chloramphenicol | (54) |
pBAD/Myc-His A | Expression plasmid replaced with chloramphenicol resistance cassette, Chloramphenicol | (11) |