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. 2022 Sep 2;8(35):eabo5506. doi: 10.1126/sciadv.abo5506

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Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 7. — (A) Actograms of VT037867-Gal4 (left), UAS-Kir2.1 (middle), and VT037867-Kir2.1 flies (right). (B) Representative weak rhythmicity (left) and arrhythmicity (right) in VT037867-Kir2.1 flies. (C) VT037867-Shibire^ts flies maintained free-running rhythms at 18°C (left) but lost free-running rhythms at 30°C (middle) and the lost free-running rhythms at 30°C recovered at 18°C (right). (D) Activity histograms of VT037867-Gal4, UAS-Kir2.1, VT037867-Kir2.1, and VT037867-Shibire^ts flies in LD. (E) DD rhythmicity statistics (period, activity, and power) of VT037867-Gal4, UAS-Kir2.1, VT037867-Kir2.1, and VT037867-Shibire^ts flies. For activity, ***P(VT037867-Gal4/VT037867-Kir2.1) < 0.001, ***P(UAS-Kir2.1/VT037867-Kir2.1) < 0.001, and ***P(18°C/30°C) < 0.001 for VT037867-Shibire^ts flies; for power, ***P(VT037867-Gal4/VT037867-Kir2.1) < 0.001, ***P(UAS-Kir2.1/VT037867-Kir2.1) < 0.001, and ***P(18°C/30°C) < 0.001 for VT037867-Shibire^ts flies. These data are analyzed by Kruskal-Wallis ANOVA with Dunn’s test. Data are expressed as means ± SEM. Actograms and histograms are averaged from four independent replicates.