Fig. 3.
Saccharomyces cerevisiae tra1Q3 mutants display phenotypes associated with increased azole sensitivity. a) tra1Q3 cells show decreased expression of genes linked to azole resistance. Wild-type and tra1Q3 C. albicans cells were incubated with 20 µg/mL fluconazole for 1 h and the expression of ERG11 and PDR5 was assessed by qRT-PCR. n = 3. b) tra1Q3 cells show increased accumulation of intracellular fluconazole. Wild-type and tra1Q3 cells were incubated with a fluorescent fluconazole probe for 1 h, and mean fluorescent intensity of intracellular fluconazole was assessed by flow cytometry. n = 4. c) tra1Q3 cells do not show increased cell death upon fluconazole treatment. Wild-type and tra1Q3 S. cerevisiae cells were incubated with 20 µg/mL fluconazole for 1 and 4 h and stained with PI to assess cell viability. Boiled cells are shown as positive control. The percentage of PI-positive cells was assessed by flow cytometry and presented in a bar graph. n = 4. All data are shown ± SEM *P < 0.05 **P<0.01.