Fig. 4.

Genome-wide screen for dosage suppressors of mtDNA stress. a) Schematic of how 3 samples were prepared for barcode sequencing screen. b) Enrichment of gene dosage increase plasmids during EtBr treatment. Rank metric is the negative, base-10 logarithm of the raw P value associated with a gene's differential enrichment multiplied by the sign of the logarithm of its fold-change (i.e. negative for plasmid depleted and positive for enriched). Genes are rank ordered along the x axis. P values calculated from 3 biological replicates. Genes for which P < 0.05 after applying Benjamini–Hochberg (BH) correction for false discovery feature magenta dots. c) Enrichment of gene dosage increase plasmids during ρ⁰ growth. Plot elements as in B, except that points are magenta for P < 10⁻⁶⁵ after BH correction. d) Comparison of EtBr and ρ⁰ growth experiments. e) Summary plot comparing ρ⁰ growth barcode sequencing screen and ρ⁰ growth rate screen results. Horizontal line indicates the growth rate of the control strain.