A. Graphical representation of data acquisition from lesional skin microarrays. B.
ZBP1 expression in lesional cutaneous lupus (CLE) (n=90) compared to healthy control (CTL) (n=13) (left), by lesion subtype (discoid lupus erythematosus (DLE) or subacute cutaneous lupus erythematosus (SCLE), middle) and based on the presence or absence of systemic lupus via >4 1997 ACR criteria (right). C. Correlation of ZBP1 expression in CLE with IFN score, linear regression. D.
ZBP1 expression in childhood onset systemic lupus erythematosus (cSLE, n=7) compared to CTL (n=8). E. Correlation of ZBP1 expression with IFN score in cSLE. F. Violin plots showing ZBP1 expression from scRNA sequencing across cutaneous cell types from nonlesional lupus skin (NLE, n=14), lesional lupus skin (LLE, n=14) compared to CTL (n=14). G. Expression of ZBP1 in adult dermatomyositis (DM) (n=41) and H. Correlation with IFN score by linear regression. I.
ZBP1 expression in juvenile dermatomyositis (jDM, n=9) compared to CTL (n=8). J. Representative images of tissue imunofluoresence of ZBP1 in CTL (n= 7), DLE (n=8), SCLE (n=5) and DM (n=6). Dotted white line indicates the dermo-epidermal junction. Scale bar =100μm. Mean + SEM. * = q < 0.05; ** = q < 0.01; *** = q < 0.0001, by Studenťs unpaired t-test.