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[Preprint]. 2024 Jan 27:2024.01.26.577394. [Version 1] doi: 10.1101/2024.01.26.577394

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Figure 1. — Integrin specificity of antibodies on all RGD-binding human and mouse integrin transfectants by indirect flow cytometry. (A) K562 stable human integrin transfectants in Ca²⁺/Mg²⁺. (B) Expi293 α5⁻/αV⁻ cell transient human integrin transfectants in Ca²⁺/Mg²⁺. (C–G) Expi293 α5⁻/αV⁻ cell transient mouse integrin transfectants in Ca²⁺/Mn²⁺. Immunostaining was performed with 50 nM IPI integrin antibody followed by washing and detection with APC-conjugated goat anti-human secondary antibodies and flow cytometry. MFI: mean fluorescence intensity.